Nissell H, Kublickas M, Lunnell NO, Petterson E. Renal function in gravidas with chronic hypertension with and without superimposed preeclampsia. Orsonneau JL, Douet P, Massoubre C, Lustenberger P, Bernard S. An improved pyrogallol red-molybdate method for determining total urinary protein. Modulo di prenotazione. Because microalbuminuria is defined as excretion of 30–300 mg of albumin/24 h (20–200 μg/min, or 30–300 μg/mg of creatinine) (20), the protein field result allows identification of microalbuminuria cases in contrast to the classic reading and reporting of the strips, which can detect only albumin concentrations 150–200 mg/L or higher (21)(22). It has been extensively evaluated for urinalysis (9)(10)(11)(15) as well as for cerebrospinal fluid analysis (16). As is the case for test strips in general, the method is based on the so-called “indicator error” principle, in which proton exchange between the indicator on the strip and the proteins in the solution produces a color change from yellow to green-blue. Colombo JP eds. Flow cytometry has been introduced for urinalysis (9)(10) to obtain quantitative data on urinary particles. 0000007901 00000 n We also wanted to explore the possibilities of combining the two novel methods, particularly in the analysis of erythrocytes, leukocytes, and glucose, which are of major clinical importance. On the basis of ROC analysis, diagnostic sensitivity was 86% (95% confidence interval, 78–92%) and specificity was 84% (95% confidence interval, 76–91%) for a reflectance value of 55.6% when the nephelometric assay was used as the comparison method at a cutoff of 30 mg/L (n = 220; 113 positive and 107 negative cases, respectively). Kouri T, Fogazzi G, Gant V, Hallander H, Hofmann W, Guder WG. We investigated the possible use of these meters to complement flow cytometry of urine and compared reflectance readings with quantitative determinations of urinary glucose and microalbumin. The role of automated urine particle flow cytometry in clinical practice. Gambke B, Kouri T, Kutter D, Nagel D, Schmid R, Scholer A. Miditron® multicenter evaluation. Regeniter A, Haenni V, Risch L, Köchli HP, Colombo JP, Frei R. Urine analysis performed by flow cytometry: reference range determination and comparison to morphological findings, dipstick chemistry and bacterial culture results – a multicenter study. questionario per l’esecuzione dell’esame citologico dell’urina (DA CONSEGNARE ASSIEME AI CAMPIONI RACCOLTI) QUESTO ESAME E’ STATO RICHIESTO DA: The dashed lines represent the lower limit of detection (− − −) with the 2 SD limits (- - - -). The correlation between WBC data (x) and leukocyte esterase measurements (y) is shown in Fig. 0000017492 00000 n Albumin in urine was measured immunonephelometrically on 220 randomly selected samples with use of commercially available Behring antibodies on a Behring Nephelometer II analyzer (Dade Behring) standardized against the widely accepted WHO/College of American Pathologists Certified Reference Material 470. The lower limit of detection (18) was calculated as the mean value − 3 SD for a blank sample. 3. TAMPONI URETRALI. 0000007964 00000 n Correlation between glucose measurements (glucose concentration different from 0) by the hexokinase-based method and glucose test strip reading (n = 85). All samples were completely processed within 2–4 h after arrival. The peroxidase-like activity of the hemoglobin-haptoglobin complex. Reproducibility of protein, leukocyte esterase, hemoglobin, and glucose on the URISYS 2400 analyzer. Controlla la creatinina e Ph. <> 0000017589 00000 n Scaderi anormale. Istruzioni per esecuzione esame urine per idoneità sportiva agonistica Compilare il modulo sotto allegato necessario per l'accettazione del campione di urina . The hemoglobin measurement is based on the peroxidase principle. Viberti GC, Jarrett RJ, Wiseman MJ. Combining diagnostic information provided by urinary flow cytometry and more quantitative test strip analysis therefore offers a theoretical basis for the development of diagnostic expert systems (11)(12). Urine per esami di citologia urinaria Sassuolo. Leukocyte esterase reflectance (y; %) = 83.7 − 15.4 log(WBC count; 106 cells/L); Spearman r = −0.688; P <0.001. Access to the instrument’s raw data theoretically allows a higher analytical sensitivity for several analytes. 0000016642 00000 n Contenitore pulito (es. xref The presence of haptoglobin in urine enhances the peroxidase activity of hemoglobin (29)(30). Does antihypertensive therapy protect the kidney in essential hypertension?. Kouri T, Kähkönen U, Malminiemi K, Vuento R, Rowan M. Evaluation of Sysmex UF-100 urine flow cytometer vs chamber counting of supravitally stained specimens and conventional bacterial cultures. The UF-100 flow cytometer automatically determines the conductivity, so we performed multiple regression analysis on the study population, calculated in a regression model the hemoglobin reflectance vs RBCs and conductivity, and found that the t-value (as expected) for RBCs was −8.442 (P <0.0001). Provetta a fondo conico, trasparente, fornita dal Laboratorio/Centro Prelievi o acquistata in Farmacia. PRELIEVI DOMICILIARI. The strips include reagent pads for ordinal scale reporting of relative density, pH, leukocyte esterase, nitrite, protein, glucose, ketones, urobilinogen, bilirubin, and hemoglobin/myoglobin. Viene venduto in scatole da 25 pezzi. In this study, we wanted to investigate the performance of quantitative urinary test strip analysis. Correlation between protein (albumin) results obtained by quantitative test strip analysis (y) and albumin (x; Behring BN II Nephelometer; n = 220). Screening and management of microalbuminuria in patients with diabetes mellitus: recommendations to the Scientific Advisory Board of the National Kidney Foundation from an ad hoc committee of the Council on Diabetes Mellitus of the National Kidney Foundation. Glucose reflectance (y; %) = 53.9 − 19.3 log(glucose; mmol/L); Spearman r = −0.851; P <0.001). The results are displayed in scattergrams, histograms, and as counts per microliter as well as counts per high-power field. The intensity of the reaction color of the test pad is detected by measuring the percentage of light reflected from the surface of the test pad. Modulo di richiesta: deve riportare Nome, Cognome, data di nascita e codice fiscale, struttura e/o Medico richiedente nonché i dati clinici essenziali (ematuria, litiasi, precedenti neoplasie vescicali, urine da catetere, da ureterostomia, da vescica ileale).. Modulo Esame Citologico. Title: Microsoft Word - Note informative Sito CdC Rizzola.doc Author: pichetti Created Date: 3/18/2020 4:11:07 PM Fair agreement was obtained between UF-100 and test strip reflectance data for erythrocytes and hemoglobin (r = −0.680) and leukocytes and leukocyte esterase (r = −0.688). 0000000752 00000 n Per effettuare l’esame urine compilare il presente modulo in ogni sua parte IN STAMPATELLO:!"!" Test strip measurements had reasonable lower limits of detection for WBC (19 × 106/L). Predicting diabetic nephropathy [Letter]. The dashed lines represent the lower limit of detection (− − −) with the 2 SD limits (- - - -). Mattenheimer H, Adams EC. • Inserire il codice esame (ECU ) e il codice richiedente 6650. 0000007922 00000 n 16 0 obj 0000028490 00000 n Correlation between RBC counts (x; flow cytometry) and test strip hemoglobin concentration (y) for RBC counts >25 × 106 cells/L (n = 96). È un’analisi che valuta caratteristiche fisiche come colore, aspetto, odore, ma anche alcune componenti chimiche come proteine, sangue e glucosio. 2. L’esame di analisi delle urine (ECMU: Esame Chimico Morfologico Urine) è un comune test di screening, non invasivo per il paziente poiché è necessario un campione delle urine che dev’essere raccolto in un contenitore sterile per la raccolta delle urine. Urine per esami di citologia urinaria Vignola. 0000029025 00000 n 0000029149 00000 n The two outer solid lines represent the 95% prediction interval around the regression line. <]/Prev 39925>> The dashed lines represent the lower limit of detection (− − −) with the 2 SD limits (- - - -). Od krvi se razlikuje po tem, da ne vsebuje krvnih celic (eritrocitov, levkocitov, trombocitov) in makromolekularnih snovi – beljakovin. Principio del test. Examenul de urina este un test de diagnostic si ajuta la identificarea substantelor si a materialului celular din urina, asociate cu diferite tulburari metabolice si renale. We also found good agreement between the hexokinase-based glucose method (x) and glucose test strip reflectance reading (y): y (%) = 53.9 − 19.3 logx (mmol/L); Spearman r = −0.851; P <0.001. 0000016774 00000 n delegato presso un qualunque centro prelievi 0 Examen de urina Pagina 8 - Reducere a capacitatii rinichilor de a asigura filtrarea si eliminarea produselor de rebut ale sangelui, de a controla echilibrul corpului in apa si saruri si de a regulariza presiunea sangvina. L'esame delle urine richiesto per la certificazione di idoneità sportiva agonistica deve essere effettuato prima della data della visita (entro i 30 giorni precedenti) utilizzando l'apposito modulo fornito dalla Società Sportiva per l'accesso al laboratorio analisi e seguendo le istruzioni. The reproducibility of urinalysis using multiple reagent test strips. (Insieme con il test viene fornito il contenitore urina da 90ml e un modulo in triplice copia).Questo Test Antidroga ha un sistema di stampa del risultato tramite il suo Strumento con software, oppure il risultato del test può essere anche scansionato o fotocopiato. /Contents 17 0 R Test strip analysis plays an important role in urinalysis as such, and the value of test strip urinalysis as a screening method has been thoroughly demonstrated (1). For WBCs, we found a lower limit of detection of 19 × 106 cells/L (CV = 1.2%). endobj x��]��q�\t�I�W�(v�q.�'{G��|�#�*�|I��V*%�4��HJ�$W����0 z�=�K�,�'K%���h�t����~�dT2�q��nrƪ�����?>�N�dn�~�����9Nn�n���31�u����� s0��f�j�}��0o��ٹ�vV:L�n�nw�~t9G/�}�-��?���zk��v����e���;�9����~�Q�x���K�#�q�xf�����˃&��x�h]�w��vJ�>Z�ڇ�B~Wٺ��7����fZ�]��U�2�� ��n�n� This is stable for 30 days when stored tightly capped at 2–8 °C. ECLM. Liquido seminale per citologia. The UF-100 measures the conductivity and categorizes the particles on the basis of their shape, size, volume, and staining characteristics. The automation of sediment analysis using a new urine flow cytometer (UF-100). 0000000015 00000 n Ottomano C. Tecnologia ed automazione dell’ esame urine quantitativo. It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide, This PDF is available to Subscribers Only. In our study correlating test strip data with urinary flow results, r was −0.69 between the flow cytometric WBC count and the leukocyte esterase reaction, although the presence of esterase inhibitors in urine and severe proteinuria might negatively affect test results for leukocyte esterase (1)(27). Per la raccolta delle urine delle 24 ore è necessario seguire scrupolosamente questa procedura: Il mattino del giorno d'inizio raccolta, scartare tutta l'urina della prima minzione mattutina, svuotando completamente la vescica nel WC. The reproducibility of (semi)automated readings is at least as good as visual readings (2), but most authors find the analytical, clinical, and labor cost-saving advantages of (semi)automated vs visual reading to be obvious (3). Viene fornito il contenitore chiuso (capacità 3 litri) ed una provetta sottovuoto tappo bianco. Examen sumar de urină Ce este examenul sumar de urină? It must be noted, however, that the sensitivity (86%) may be inadequate for patients with known diabetes in whom physicians do not want to miss microalbuminuria in their annual testing. The UF-100 automatically detects and counts red blood cells (RBCs), white blood cells (WBCs), bacteria, yeast cells, crystals, epithelial cells, small round cells, sperm cells, and casts. Because microalbuminuria is characterized by large intraindividual variability, an ordinal-scale answer might be misleading. BREATH TEST. >> Whelan PV, McGill C, Ng K. Evaluation of the BMC Chemstrip urine analyzer and Chemstrip 10 UA test strip with emphasis on comparison with analytical, chemical, physical, and microscopic methods [Abstract]. This implies that the test has the potential to offer a screening for microalbuminuria without increased cost, hence coming closer to the “urine test strip of the future” (23). Alkalinization of urine gives rise to erroneous results in particle counting. The lower limit of detection was 25 mg/L (CV = 2.2%) (18). Opis fizičkih i fizičko-hemijskih karakteristika mokraće: izgled, boja, miris, reakcija i specifična težina. Results: Reproducibility of the reflectance signal was good for high- and low-concentration urine pools for protein (0.8% and 0.9% and 1.5% and 2.2% within and between runs, respectively), leukocyte esterase (1.1% and 1.0%; 5.1% and 1.2%), hemoglobin (1.7% and 1.1%; 8.9% and 1.1%) and glucose (2.1% and 0.5%; 6.5% and 2.3%). European urinalysis guidelines. Appunti per la prova scritta ed orale dell’esame di inglese, modulo 1, 2 e 3 previsto dal nuovo ordinamento per la facoltà di medicina e chirurgia per gli studenti del triennio biologico. Esame delle urine: si cambia Published in: La Rivista Italiana della Medicina di Laboratorio, July 2017 DOI: 10.1007/s13631-017-0157-7: Authors: Rudi Ravasio, Giulia Previtali, Francesca Nembrini, Maria Grazia Alessio View on publisher site Alert me about new mentions. Test strip analysis plays an important role in urinalysis as such, and the value of test strip urinalysis as a screening method has been thoroughly demonstrated (1). It is known that reducing substances (e.g., ascorbic acid) may lower the signal, whereas oxidizing substances may have a positive effect on measured hemoglobin concentration. 3. 0000017717 00000 n Data are expressed in an ordinal scale (as “normal”, “negative”, “positive”, or as nominal concentrations) on the reports, but (quantitative) reflectance data can be downloaded to floppy disks. TEST DI MEARES. Urin je ultrafiltrat plazme in ga tvorijo ledvice. A lower limit of detection of 8 × 106 cells/L (CV = 1.2%) was calculated. Raccogliere le prime urine del mattino o, in alternativa, le urine emesse dopo almeno 2 ore dalla prima. La provetta contenente le prime urine della mattina deve essere portata e consegnata dall'utente o da suo? Per informazioni e/o chiarimenti rivolgersi a : segreteria U.O. Recently, the URISYS 2400 automated urine test strip analyzer (Roche Diagnostics) was introduced. It therefore is advantageous to have a better estimation of the true concentration. L’esame delle urine è tra le prime indagini richieste per la valutazione della funzione renale. <> 0000016709 00000 n 0000000868 00000 n Urine puternic acide (pH < 4,5) se produc in procesele maligne (are loc distrugerea crescuta de proteine), febra, diaree abundenta, acidoza diabetica sau metabolica. We studied 436 freshly collected urine samples submitted to our routine laboratory for diagnostic urinalysis. Multiple regression analysis was used to investigate a model relating leukocyte esterase and hemoglobin field reflectance. In this study, we compared the URISYS 2400 automated strip reader with the Sysmex UF-100 flow cytometer to evaluate the possible value of quantitative test strip data in a urinary expert system. The lower limits of detection for erythrocytes and leukocytes were 8 × 106/L and 19 × 106/L, respectively. 1. Fenili D, Pirovano B. Ultimo aggiornamento: 19 Luglio 2019 Condividi. In conclusion, quantitative urine test strip analysis provides reliable data on WBCs, RBCs, glucose, and albumin. Particles that cannot be classified in one of the former categories are counted as “other cells”. SPERMIOGRAMMA. 0000001055 00000 n • Applicare a lato un’etichetta con il codice a barre. 2. The detection limit of the URISYS 2400 protein assay was 25 mg/L if restricted to albumin alone and total protein was not considered. La documentazione di riferimento per l‟esecuzione dell‟esame urine dovrebbe comprendere una accurata descrizione della fase preanalitica, analitica e post-analitica. In particular, we wanted to compare the reflectance readings of the protein test field with immunochemical microalbumin determinations. Because microalbuminuria is generally regarded as an excellent marker for assessing early renal damage in common conditions such as diabetes and hypertension (4)(5)(6)(7) and as an early predictor of preeclampsia during pregnancy (8), the availability of highly sensitive test strip readers opens interesting perspectives for assessing this phenomenon. The principle is based on argon laser flow cytometry. This leads to improved elimination of occasional errors in the WBC and RBC counting channels of the flow cytometer (11). Najčešće hemijske analize su analize na proteine, šećer, ketonska tela, urobilinogen, bilirubin i nitrite. Langlois et al. Tutela della salute nelle attività sportive, tel. Modulo di prenotazione. 15 0 obj Modde H, Rodriguez S. Urin Mikrobiologie. Istruzioni per la raccolta delle urine per l'esame delle 24 ore. Urine per esami di citologia urinaria raccolte Castelfranco Emilia. 0000016847 00000 n Modulo di prenotazione. Two discrepancies (0.9%) were found in which the test strips on both automated strip readers overestimated the urinary albumin concentration by >10-fold. Delanghe JR, Kouri TT, Huber AR, Hannemann-Pohl K, Guder WG, Lun A, et al. Van Acker J, Delanghe J, Langlois M, Taes Y, De Buyzere M, Verstraete G. Automated flow cytometric analysis of cerebrospinal fluid. For full access to this pdf, sign in to an existing account, or purchase an annual subscription. Valori scazute ale pH-ului urinar (deci urine acide) se intalnesc in: Reumatismul poliarticular cronic; ESAME URINE PER IDONEITA’ AGONISTICA MEDICINA DELLO SPORT ... Consegnare il presente modulo e la provetta con le urine (almeno 4 giorni lavorativi prima della data della visita medico sportiva ) presso uno dei seguenti Centri Prelievo, nei giorni e negli orari indicati.