This overlapping may lead to a certain level of subjectivity, as reported by some authors (19, 20). A systematic literature search was conducted to target all scientific publications related to the evaluation of p16INK4a immunoreactivity versus HPV testing and p53 immunoreactivity in vulvar diseases (VIN and VSCC) from January 2004 to December 2016. One is associated with high-risk human papillomavirus (hrHPV) infection and presents with multifocal VIN (warty or basaloid types). Devo ringraziare le persone che me l'hanno consigliata perchè mi hanno permesso di conoscere una persona splendida sotto ogni punto di vista, sia umano che professionale. The incidence of HPV-related VIN is increasing worldwide and is implicated in carcinogenesis. View or download all the content the society has access to. PubMed (MEDLINE), EMBASE, the Cochrane Library, Scopus as well as Clincaltrials.gov were systematically and independently screened by 2 authors (Carrone A, Savone D) and then compared to identify the eligible studies. By examining each work by its salient characteristics, we can highlight the following findings. Avvocato PICCIANO MARIA GRAZIA. We found that nuclear and cytoplasmic immunostaining for p16INK4a was intense and diffuse in HPV-associated lesions and weak and focal in normal vulvar epithelium, nondysplastic lesions, lichen sclerosus and keratinizing vulvar squamous cell carcinoma. Per ulteriori informazioni contattaci. Od 1990. je radila kao manekenka prije nego što je pažnju svjetske javnosti privukla u filmu Il Postino (Poštar), tumačeći ulogu lokalne ljepotice u koju se zaljubi glavni junak.. Izvori Scopri un sorriso anche con Maria Grazia. +2 Altro. In specimens of keratinizing VSCC, HPV positivity ranged from 1.3 to 13.3% and p53 immunostaining was positive in 64.5% of cases where evaluated. Angela Carrone, Lucia Riganelli, Delia Savone. IARC. Dott.ssa Maria Grazia Carrara, Faculty of Statistics Department of Statistical Sciences, Università degli Studi di Milan-Bicocca. Yelp is a fun and easy way to find, recommend and talk about what’s great and not so great in Catania and beyond. 2 Department of Gynecological, Obstetrical and Urological Sciences, “Sapienza” University and Umberto I Polyclinic of Rome, Rome - Italy See all articles by this author. Immunohistochemistry was performed in 3,110 specimens with a histological diagnosis of VIN and VSCC, including also 2% (n = 63) of samples of normal epithelium or nondysplastic lesions (hyperplasia, lichen sclerosus, lichen simplex chronicus, seborrheic keratosis, condylomas). Management of HPV-Related Cervical Disease: Role of p16INK4a Immunochemistry. Vulvar squamous cell carcinoma (VSCC) accounts for more than 90% of malignant tumors of the vulva (3), with 2 different etiopathogenic pathways for its development. Per contattarmi puoi chiamare il numero +39 347 7868495 mandarmi una email. pp. The last items were the outcomes explaining HPV-detection prevalence in association with the immunoreactivity used and the microscopic assessment cutoffs identified for each study to determine the positivity or negativity of the specimens. Simply select your manager software from the list below and click on download. If you have access to a journal via a society or association membership, please browse to your society journal, select an article to view, and follow the instructions in this box. Contact us if you experience any difficulty logging in. In the study by Santos et al (11), p16INK4a positivity was established when sheets or large clusters of contiguous positive cells representing more than 25% of epithelium were identifiable; also p53 nuclear immunostaining was considered positive when found in 25% or more of cells. Most of them showed close areas positive for squamous cell carcinoma (SCC). Rufforny et al (16) in 2005 reported the different expression pattens of p16 for benign vulvar lesions, VIN1, VIN2, VIN3 and VSCC, concluding that in benign cases and in condylomatous lesions, p16INK4a and HPV DNA (generally the HPV16 genotype) showed no expression, thereby demonstrating the usefulness of p16INK4a in premalignant and malignant lesions. Watch Queue Queue. Join Facebook to connect with Mariagrazia Piccioni and others you may know. All included studies allowed comparison of immunochemistry triage with HPV DNA PCR test. By continuing to browse The triage tests were itemized with the commercial triage methods used for each study. Maria Grazia Cucinotta (Messina, 27. jul 1968) je italijanska glumica. Basic statistical analysis was conducted using common methods of descriptive statistics with assessment of arithmetic mean, minimum and maximum values and geometric mean to evaluate the sensitivity and specificity percentages of p16INK4a and HPV DNA, p53 and Ki67. : 335 60 94 596 Tel. Curriculum vitae download. HrHPV-associated premalignant lesions and carcinomas showed intense and diffuse nuclear and cytoplasmic immunostaining for p16INK4a, while no expression of p16INK4a or heterogeneous expression was found in normal vulvar epithelium and nondysplastic lesions. This is in agreement with the findings of Rufforny et al (16), who demonstrated that HPV-related VIN is a “younger” pathology than HPV-negative vulvar tumors. Invece la Dott.ssa e' la luce per tutte noi. Il mio studio privato è in via I. Alvisi 16 a Barletta. 415.8k Followers, 630 Following, 2,304 Posts - See Instagram photos and videos from MariaGraziaCucinotta Official (@maria_grazia_cucinotta) Therefore, the clear recognition of HPV-related lesions should lead to an appropriate strategy of treatment and follow-up. The characteristics and technical information of these 8 studies are listed in Table I. +39 339 44 44 247 Casa Piccioni Strada Vecchia Passera-Vedasco 6 28838 Stresa Frazione Passera Italia HPV-related carcinogenesis is characterized by hrHPV DNA integration into the host DNA (10). We included all English-language retrospective and prospective studies at primary diagnosis assessing p16INK4a immunostaining or p16INK4a/p53 staining, with HPV DNA testing (HC2/PCR) as the comparator test, on histological specimens of VIN and VSCC verified with a reference standard. Questo sito utilizza cookie tecnici e di profilazione propri e di terze parti per le sue funzionalità e per inviarti pubblicità e servizi in linea con le tue preferenze. This site uses cookies. a case-control study: GYNECOLOGICAL ENDOCRINOLOGY: 2020: HPV DNA positivity also was distinguishable between uVIN and dVIN: the first type showed a significantly higher percentage (84%) of hrHPV positivity (also if adjacent SCC areas were present), while 100% of dVIN cases were hrHPV negative. Like in cervical immunochemistry, p16INK4a or p16INK4a/p53 or p16INK4a/Ki67 dual staining could improve the identification of uVIN and increase the sensitivity of histological interpretation. The positivity criteria for p16INK4a immunostaining were highly homogeneous in all studies: strong nuclear and cytoplasmic staining in the basal and higher layers of epithelium was considered positive for p16INK4a expression. Maria Grazia Piccioni Department of Gynecological, Obstetrical and Urological Sciences, “Sapienza” University and Umberto I Polyclinic of Rome, Rome - Italy See all articles by this author. l meglio: Ho apprezzato molto il fatto che facesse molte domande, proprio per essere sicura di aver preso in considerazione tutte le ipotesi. Cases of invasive vulvar cancer (IVC) and vulvar intraepithelial neoplasia (VIN) have been rising recently among younger women and the rates of new vulvar cancer events have increased by 0.5% each year over the last 10 years (2, 3). In specimens of warty/basaloid VSCC, HPV positivity ranged from 62.5% to 100%, p16INK4a immunoreactivity from 76.4% to 100%, and p53 immunostaining was positive in 6.2% of cases. As in cervical cancer, p16INK4a is a marker of HPV integration into the host genome and a useful tool to classify VIN into hrHPV-associated and hrHPV-independent vulvar lesions. LEARN MORE. Maria Grazia Misuriello Psicologa Psicoterapeuta Ordine degli psicologi Regione Puglia n 1696. Reuschenbach et al (18) also evaluated VSCC based on HPV DNA, p16INK4a and p53 expression, with tumor location limited to the anterior vulvar fourchette. The remaining 8 studies were included in the review. 5 recensioni, La Professoressa Maria e' meravigliosa. A difference in patient age was highlighted: in women with uVIN with adjacent SCC areas the median age was lower (52 years, standard deviation [SD] 13.4 years) than in those with dVIN with associated SCC (74 years, SD 12.5 years). Sono occupata anche in ambito accademico: sono infatti docente presso il Corso di laurea in Medicina e Chirurgia per la materia di Ostetricia e Ginecologia presso l'Università "La Sapienza" di Roma, nella quale io stessa mi sono lauerata. Scopri quali alberi ha e tutto quello che sta facendo per rendere il mondo più verde. Contatti. Contatti. Patient age was unremarkable, with some exceptions: the mean age of women with HPV16 positivity (and the relative E6/E7 transcription) was significantly lower (p<0.001) than that of women with vulvar cancers not linked to HPV DNA. Il tuo messaggio. Table I Details and findings of included studies. Ecografia ostetrica Search Google Scholar for this author, Felice Patacchiola, MD 4. Also, Ki67 immunostaining showed high, diffuse and nuclear positivity in uVIN, while it was absent or confined to the parabasal epithelial layer in dVIN and it was absent in normal epithelium. However, its expression in vulvar carcinomas, which have a diverse pathogenesis, has not been extensively studied and only a few studies were reported in the scientific literature. La tua email. I have read and accept the terms and conditions, View permissions information for this article. In questo periodo in cui i pazienti presentano scarsa fiducia nella sanità 37-38. Visita ginecologica + ecografia pelvica + pap-test a Roma, Malattia sessualmente trasmissibile a Roma, Ginecologi con Allianz global assistance (mondial assistance ) a Roma, Ginecologi con Unipol assicurazioni (unisalute) a Roma. Maria Grazia Piccioni is on Facebook. Cancer incidence in five continents, Probable nonpapillomavirus etiology of squamous cell carcinoma of the vulva in older women: a clinicopathologic study using in situ hybridization and polymerase chain reaction, Recent advances in the pathology of the vulva, Squamous vulvar intraepithelial neoplasia: 2004 modified terminology, ISSVD Vulvar Oncology Subcommittee, The etiologic role of HPV in vulvar squamous cell carcinoma fine tuned. Maria Grazia Cucinotta, Actress: The World Is Not Enough. View the profiles of people named Maria Grazia Pacchioni. voglio mettere in evidenza che ci sono medici che svolgono il loro lavoro con dedizione e passione anche quando vengono sopraffatti dai ritmi quotidiani e dal mal funzionamento organizzativo. Differently from dVIN, there is no agreement on the gold-standard treatment for uVIN, which often presents multicentric and multifocal involvement. For more information view the SAGE Journals Sharing page. dVIN was always HPV and p16INK4a negative, while p53 immunostaining was detected in 84%-90.8% of cases and MIB1 immunostaining was low in 96% of cases. MILANETTI ALESSANDRO . The role of p16INK4a expression in uterine cervical cancer is well established. Visualizza il profilo di Maria Grazia Picchioni su LinkedIn, la più grande comunità professionale al mondo. 570 d. vind-ik-leuks. In particular, a retrospective cross-sectional study including 39 countries (14) evaluated HPV DNA and p16INK4a levels by PCR and immunochemistry, respectively. This product could help you, Accessing resources off campus can be a challenge. Join Facebook to connect with Maria Grazia Piccioni and others you may know. For more information view the SAGE Journals Article Sharing page. Additionally, women with uVIN and no SCC areas had a still lower median age at the time of diagnosis: 36 years (SD 10.8 years). Four studies evaluated sample data and percentages for uVIN and dVIN (11, 13–14–15); the other 4 also considered immunostaining in normal epithelium, benign lesions, VIN and VSCC, without distinguishing between uVIN and dVIN (8, 16–17–18). Adi Shakti - Bhakti Mantra - Duration: 12:43. The evaluated studies showed comparable p16INK4a immunoreactivity and HPV positivity in HPV-related uVIN. Discrepancies were not infrequent in this study. Lee et al (25) evaluated the presence of HPV or its surrogate, p16 immunostaining, as an independent prognostic factor for in-field relapse and survival in women with VSCC treated with radiotherapy. E' stata molto gentile ed umana e posso dire di essere stata visitata da una persona esperta e competenta. Members of _ can log in with their society credentials below. Search Google Scholar for this author, Pierluigi Benedetti Panici. Nel dramma la Ginzburg disegna il ritratto di un matrimonio, di carattere piuttosto borghese, tra l'avvocato Pietro e Giuliana, una giovane donna di bassa estrazione sociale conosciuta ad una festa.